USB killer is a device that looks similar to a normal USB, when attached to a USB port of any device (PC, laptop, network device, smart TV), will fry up their components causing massive damage.
To clearly understand how it works we need to define a couple of terms:
USB killer device consists of high capacity capacitors which charge very quickly. So when it is inserted in a USB port of host devices, it draws power from the port and charges the capacitors to around 100~200 volts.
Once capacitors are fully charged it discharges (sends electric shock) this current directly to the data pins of the USB port—which in turn is connected to motherboard/main circuitry of PC/device.
Typical a USB port rating is 5 V, 1 A, and the Power transferred by the USB killer to port = 200 V and few mill ampere currents.
This process of charging and discharging (sending high voltage shock) keeps repeating until USB port(along with another part) gets damaged & stops supplying power to the USB killer.
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The USB Killer Shield is the way to safely use and test unknown USB devices. Protect your computer and hardware – always use a USB Killer Shield before trusting a new device.
Protecting your hardware is easy: The Shield allows power to travel through the device but will block any Power Surge attacks.
It also provides break-out cables to test if a power surge is present. Once verified, you can directly plug in the device, with 100% certainty.
Another way is to use LINDY USB Port Lock & Blocker, the following video describes its functionality.